
Jurgen Sygusch
Analyse structurale des macromolécules et signalisation cellulaire
Profile
Research expertise
Function of the molecular architecture in enzymatic catalysis, macromolecular interactions and design of inhibitors
To understand how proteins and enzymes function at the molecular level and to translate this knowledge to disease states, we have focused our study on selective macromolecules functioning in metabolism and plant defence. The central metabolic pathway of glycolysis which is found in all living organisms converts glucose to metabolic intermediates that generate energy in form of ATP for cellular functions. The glycolytic enzyme we have studied most intensively is fructose 1,6-bisphosphate aldolase which cleaves a very specific C-C bond that is considered generally difficult to perform chemically. Aldolase has proven to be a rich source of fundamental knowledge on how enzymes perform their catalytic function and is casting a new perspective on the role of glycolysis in various cellular mechanisms. We are examining in particular the role of this enzyme in a number of diseases that involve extensive glucose utilization; these include cancer, protozoan infections (malaria, sleeping sickness, Leishmanias) and type II diabetes. Toxic secondary metabolites are generated during glycolysis which modifies cellular proteins and these modifications have been implicated in a number of pathologies such as diabetes. Deglycation enzymes exist that can undo these types of modifications at the molecular level and are being investigated. Also of interest are related enzymes that cleave the same C-C bond however recognizing substrates that have subtly different substituent geometries about the cleaved bond. Understanding this differential cleavage activity has important application in controlling bacterial virulence. A more recent interest has been the DNA binding activity of plant transcriptional factors to improve plant resistance to disease. The experimental techniques we routinely use to explore biological structure at the molecular level are protein crystallography, molecular dynamics, and enzymology. A long standing interest has been the application of microgravity to protein crystal growth in order to alleviate this bottleneck in crystallographic studies. Protein purification, gene cloning and expression, site directed mutagenesis, enzyme kinetics, bioinformatics and cell culture are also experimental tools we use in our research.Teaching and supervision
Teaching
Courses taught (current session only)
Programs
Student supervision
Theses and dissertation supervision (Papyrus Institutional Repository)
Mechanism of N-Type Inactivation in Shaker Potassium Channels
Cycle : Doctoral
Grade : Ph. D.
Studying biological assembly of ion channel complexes
Cycle : Doctoral
Grade : Ph. D.
Structure-function studies of class I aldolases - exploring novel activities : mechanism, moonlighting, and inhibition
Cycle : Doctoral
Grade : Ph. D.
Fructose-1,6-bisphosphate aldolase de classe II : aspects structural et dynamique dans le mécanisme réactionnel
Cycle : Master's
Grade : M. Sc.
La tagatose-1,6-bisphosphate aldolase et la fructose-1,6-bisphosphate aldolase de classe I : mécanisme et stéréospécificité
Cycle : Doctoral
Grade : Ph. D.
Étude structuro-fonctionnelle de SecP43 et caractérisation de ses interactions avec SepSecS et l’ARNtSec in vitro
Cycle : Master's
Grade : M. Sc.
Étude structurale du mode de liaison des protéines Whirly de plantes à l’ADN monocaténaire
Cycle : Doctoral
Grade : Ph. D.
Étude structure-fonction des fructose-1,6-bisphosphate aldolases métallo-dépendantes : mécanisme catalytique et développement d’antimicrobiens
Cycle : Doctoral
Grade : Ph. D.
Caractérisation structurale et fonctionnelle des interactions impliquant TFIIH et la machinerie de réparation de l’ADN
Cycle : Doctoral
Grade : Ph. D.
Étude structurale de la fructoselysine 6-kinase d’Escherichia coli : reconnaissance de substrats et mécanisme enzymatique
Cycle : Master's
Grade : M. Sc.
Structure d'une tagatose-1,6-bisphosphate aldolase de classe I : étude d'une apparente perte de stéréospécificité
Cycle : Master's
Grade : M. Sc.
Multifonctionnalité de l'aldolase glycolytique : mécanisme catalytique et interaction avec un peptide de la protéine du syndrome Wiskott-Aldrich
Cycle : Doctoral
Grade : Ph. D.
Intermédiaires réactionnels de la fructose-1,6-biphosphate aldolase de Leishmania mexicana : comparaison avec l'enzyme de mammifère et recrutement de la région C-terminale
Cycle : Master's
Grade : M. Sc.
La fructose-1,6-bisphosphate aldolase de Thermus aquaticus : séquence du gène, caractérisation et cristallisation de la protéine surexprimée
Cycle : Master's
Grade : M. Sc.
Structure d'un intermédiaire réactionnel covalent de la 2-kéto-3-déosxy-6-phosphogluconate aldolase d'E.coli à une résolution de 1.95Å
Cycle : Master's
Grade : M. Sc.
Détermination du mécanisme catalytique de l'aldolase du muscle de lapin recombinante à partir de l'étude structurale et cinétique de certains de ses mutants
Cycle : Doctoral
Grade : Ph. D.
Détermination du rôle de résidus clés du site actif dans le mécanisme catalytique de l'aldolase de muscle de lapin recombinante
Cycle : Master's
Grade : M. Sc.
Projects
Research projects
Structural investigation of reaction intermediates in glycolytic aldolases
Supplément COVID-19 CRSNG_Structural investigation of reaction intermediates in glycolytic aldolases
Groupe d'étude des protéines membranaires (GEPROM) Cryo-electron microscopy of the bacterial membrane complexes TriABC and MmpL3
MmpL3 assays and structure
ROBOT FOR LIPIDIC CUBIC PHASE (LCP) CRYSTALLIZATION OF MEMBRANE PROTEINS
Training program in cellular dynamics of macromolecular complexes
STRUCTURAL INVESTIGATION OF REACTION INTERMEDIATES IN GLYCOLYTIC ALDOLASES
LOW-VOLUME ITC FOR INVESTIGATING BIOMOLECULAR INTERACTIONS
STRUCTURAL INVESTIGATION OF REACTION INTERMEDIATES IN GLYCOLYTIC ALDOLASES
PROTEIN SECRETION AND INTRACELLULAR SURVIVAL BY TOXOPLASMA
MACROMOLECULAR CRYSTALLIZATION FACILITY
Publications and presentations
Publications
- Pour consulter les publications du Dr. Jurgen Sygusch : http://esilrch1.esi.umontreal.ca/~syguschj/publication.html
Disciplines
- Cell Biology
- Biochemistry
- Molecular Biology
Areas of expertise
- Nucleic Acids
- Biotechnology
- Cell
- Combinatorial Chemistry
- Chromosome (Living Organisms)
- Inorganic and Organometallic Compounds
- Enzymes and Proteins
- Gene (Living Organisms)
- Macromolecules
- Biological and Biochemical Mechanisms
- Bioactive Molecules
- Organic Molecules and Biomolecules
- Cell Signaling (Circulatory and Respiratory Health)
- Chemical Synthesis and Catalysis
- Biomedical Technologies
- COVID-19
- COVID19
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