Jean-François Côté
Cancer: organisation du cytosquelette et migration cellulaire
- Professeur/chercheur titulaire
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Faculté de médecine - Département de médecine
- Professeur accrédité
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Faculté de médecine - Département de biochimie et médecine moléculaire
Profile
Research expertise
Dr. Côté’s group focuses on understanding the role of the DOCK180/ELMO-Rac pathway in cell migration using biochemical, cellular, genetic and structural approaches. We are currently studying the molecular players involved in myoblast fusion during the formation of skeletal muscle in order to identify the biological mechanisms regulated through DOCK180/Rac. In addition, we are interested in understanding the molecular mechanisms controlling signaling by receptor tyrosine kinases in the induction of cell invasion and metastasis. In vivo models are used to study the role of AXL and HER-2 in the progression of breast cancer.
Biography
Fonctions actuelles
- Directeur, unité de recherche sur l’organisation du cytosquelette et la migration cellulaire, Institut recherches cliniques de Montréal (IRCM)
- Professeur agrégé de recherche IRCM
- Directeur associé aux affaires académiques affaires étudiantes)
- Professeur-chercheur agrégé, Département de médecine (accréditation en biologie moléculaire), Université de Montréal
- Professeur associé, Département d'anatomie et biologie cellulaire, Université McGill
- Chercheur-boursier, Fonds de recherche du Québec - Santé
education
- 2005 — Postdoctorat — Biologie cellulaire — The Sanford Burnham Medical Research Institute
- 2001 — Doctorat — Biochimie — Université McGill
- 1996 — Maîtrise (Sciences expérimentales de la santé) — Biologie et autres sciences connexes — INRS Armand-Frappier
- 1994 — Baccalauréat — Biochimie — Université de Montréal
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Affiliations and responsabilities
Research affiliations
Research units
Membre
Affiliated institutions
- Institut de recherches cliniques de Montréal (IRCM)
Teaching and supervision
Student supervision
Theses and dissertation supervision (Papyrus Institutional Repository)
Rôle de GAS6 et de son récepteur AXL dans la dérégulation de l’homéostasie glucidique et le développement de l’insulino-résistance
Cycle : Doctoral
Grade : Ph. D.
Genomic approaches to determine genes that regulate breast cancer metastatic dormancy and relapse
Cycle : Doctoral
Grade : Ph. D.
Efficient reformulations for deterministic and choice-based network design problems
Cycle : Master's
Grade : M. Sc.
Rôle du récepteur tyrosine kinase AXL dans la progression métastatique du cancer du sein
Cycle : Doctoral
Grade : Ph. D.
Establishing a role for the scaffold proteins Tanc1 and Tanc2 in myoblast fusion
Cycle : Doctoral
Grade : Ph. D.
Impact de la régulation conformationnelle des protéines Elmo sur le muscle squelettique et les maladies
Cycle : Doctoral
Grade : Ph. D.
AXL receptor tyrosine kinase in breast cancer : defining novel substrates and pathways involved in cell motility and invasion
Cycle : Doctoral
Grade : Ph. D.
Dissection de la fonction du RCPG d'adhésion BAI3 dans la fusion des myoblastes
Cycle : Doctoral
Grade : Ph. D.
Étude sur les fonctions in vivo des GEFs DOCK chez les mammifères
Cycle : Doctoral
Grade : Ph. D.
Exploring Rac GTPase regulation : the molecular mechanisms governing the DOCK180 and ELMO interaction and the role of this complex in Rac-mediated cell migration
Graduate : Patel, Manishha
Cycle : Doctoral
Grade : Ph. D.
Étude des voies de signalisation en amont et en aval de la petite GTPase Rac1
Graduate : Pelletier, Ariane
Cycle : Master's
Grade : M. Sc.
Étude du rôle de l'adaptateur Nck2 dans la progression métastatique du mélanome humain
Graduate : Labelle-Côté, Mélissa
Cycle : Master's
Grade : M. Sc.
Projects
Research projects
How do RHO GEFs achieve specific signalling?
Canada Research Chair in Cellular Signalling and Cancer Metastasis
Unravelling the mechanisms of AXL trafficking and signalling in breast cancer metastasis
Systematic mapping of the global ARF network interactome by BioID coupled to functional studies to reveal novel biological functions
Role of PAK2 in tumor angiogenesis and in the tumor microenvironment
Deciphering the signalling cascades controlling cell-cell fusion
A novel proteomics approach to uncover Rho GTPases effector pathways
Mechanisms of breast cancer metastasis: Understanding the roles and molecular functions of the RTK AXL
Supplément COVID-19 CRSNG_A novel proteomics approach to uncover Rho GTPases effector pathways
Targeting the HectD1/ACF7 signalling axis in breast cancer progression
Role and regulation of PAK2 in endothelial cells and in tumour angiogenesis
Defining the contribution of the microtubule +TIP-binding protein ACF7 toward breast cancer progression
Role of PAK2 in angiopoietins/Tie2 signaling in endothelial cells and in angiogenesis
CONTRÔLE DE LA SIGNALISATION PAR LA PETITE GTPASE RAC: IMPLICATIONS LORS DU DEVELOPPEMENT MUSCULAIRE ET DANS LE PROCESSUS METASTATIQUE
Investigating the roles of a subset of oncogen-induced interferon Type 1 genes ine HER2 breast cancer metastasis
Signaling via microtubule dynamics during HER2-mediated breast cancer metastasis
Description
Metastasis is a pathological process whereby breast tumor cells acquire the ability to leave the mammary gland to explore the body and, ultimately, colonize other organs. It is such secondary tumor sites, often far away from the initial breast tumor, that pose a menace to the life of breast cancer patients. Our lab is committed to uncover the molecular mechanisms that facilitate both the escape of cancer cells from the breast tumors and metastasis with the goal of identifying new therapeutic targets for the treatment of patients. In this application, we focus on a protein complex that enables cell migration, a key biological process for metastasis. In the first part of our program, we use a cellular system to dissect how a potential mediator of metastasis, ACF7, is turned on/off. In the second part, we use engineered mice that develop breast cancer to test if this ACF7 protein is essential for metastasis of the breast cancer cells to the lungs of mice. Our program may uncover a signaling hub that could be exploited for therapeutic approaches that would limit metastasis.
SIGNALING VIA MICROTUBULE DYNAMICS DURING HER2-MEDIATED BREAST CANCER CELL METASTASIS
Mechanism of breast cancer metastasis: AXL signaling in the tumoral microenvironment
Description
The most important clinical factor associated with poor survival during breast cancer progression is the appearance of metastasis. It is such secondary tumor sites, often far away from the initial breast tumor, that threaten the life of afflicted women. Our research program aims at uncovering the molecular mechanisms that facilitate both the escape of cancer cells from the breast tumor and their ability to enter blood vessels to ultimately travel to secondary tumor soil where they can grow as metastasis. Dr. Côté and his team focus on a protein named AXL that is found at the surface of both the cancer cells and the tumor blood vessels. The presence of AXL in human breast tumors correlates with the formation of metastases. Here, they propose to study how AXL is working in both the tumor cells, to promote their motility, and in the blood vessels, to facilitate the seeding of the tumor cells at distant sites. To achieve these objectives, they will use engineered mice that recapitulate human breast cancer in addition to cell culture models to study cell movement and blood vessel integrity.
Structural and functional studies to elucidate the role of DOCK 180 pathway in cell polarity, myoblast fusion and podocyte integrity
STRUCTURAL AND FUNCTIONAL STUDIES TO ELUCIDATE THE ROLE OF THE DOCK180 PATHWAY IN CELL POLARITY, MY OBLAST FUSION AND PODOCYTE INTEGRITY
CANCER DU SEIN ET METASTASES : ROLES DE LA SIGNALISATION EMANANT DE LA KINASE AXL DANS LE MICRO-ENVIRONNEMENT TUMORAL
Contrôle de la signalisation par la petite GTPase Rac lors du développement embryonnaire et dans le processus métastatique
Description
CONTRÔLE DE LA SIGNALISATION PAR LA PETITE GTPASE RAC LORS DU DEVELOPPEMENT EMBRYONNAIRE ET DANS LE PROCESSUS METASTATIQUE
ERBB-2 SIGNALING TO THE RAC GTPASE IN BREAST CANCER METASTASIS : ROLE OF THE DOCK180-ELMO COMPLEX
Research exploitation
Colloque Signalisation Québec 2012
Description
Organisation du Colloque Signalisation Québec 2012, 13 au 15 juin 2012.
Outreach
Publications and presentations
Publications
- Mélanie Laurin, Jennifer Huber, Ariane Pelletier, Tarek Houalla, Morag Park, Yoshinori Fukui, Benjamin Haibe-Kains, William J. Muller and Jean-François Côté. “The Rac-specific guanine nucleotide exchange factor DOCK1 is a critical regulator of HER2-mediated breast cancer metastasis”. (2013) P.N.A.S.; in revision.
- Yoran Margaron, Nadine Fradet, and Jean-François Côté. “ELMO recruits Actin Crosslinking Family 7 (ACF7) at the cell membrane for microtubule capture and stabilization of cellular protrusions”. (2013) J Biol Chem, 288(2), p1184-1199.
- Manishha Patel*, Yoran Margaron*, Nadine Fradet, Qi Yang, Brian Wilkes, Michel Bouvier, Kay Hofmann and Jean-François Côté. “An evolutionarily conserved autoinhibitory molecular switch in ELMO proteins regulates Rac signaling”. (2010) Current Biology, 20(22), p2021-2027.
- Jean-François Côté* and Kristiina Vuori*. “Cell Biology. Two Lipids That Give Direction.” (2009) Science, 324(5925), p346-347.
- Mélanie Laurin, Nadine Fradet, Anne Blangy, Alan Hall, Kristiina Vuori* and Jean-François Côté*. “The atypical Rac activator Dock180 (Dock1) regulates myoblast fusion in vivo” (2008) P.N.A.S., 105(40), p15446-15451.
- Jean-François Côté, Andrea B. Motoyama, Jason A. Bush and Kristiina Vuori. “A novel and evolutionarily conserved PtdIns(3,4,5)P3-binding domain is necessary for DOCK180 signalling” (2005) Nature Cell Biology, 7(8), p797-807.
Disciplines
- Cell Biology
- Molecular Biology
- Oncology
Areas of expertise
- Apoptosis and Cancer
- Breast Cancer
- Embryonic Development
- Muscular Diseases
- Cell Signaling and Cancer