Health Sciences
Puttaswamy Manjunath
Biochimie et spermatozoïdes
Secondary numbers:
514 252-3562
(Travail 1)
514 252-3430
(Télécopieur)
Profile
Research expertise
Biochemical characterization and physiological role(s) of proteins from the male reproductive tract:
In mammals, sperm emerging from the male reproductive tract are incapable of fertilizing egg; however, they acquire this ability during their journey in the female reproductive tract. This prerequisite conditioning is called capacitation and it is one of the key events that regulate fertility. A part of this process involves loss or modification of surface components acquired by sperm during epididymal maturation and their contact with seminal plasma. We have isolated and characterized a family of proteins from bovine seminal plasma (BSP), which are added to sperm during ejaculation. Our studies have demonstrated that these proteins are essential for capacitation and are ubiquitous in mammals. Sequences homologous to BSP proteins have been identified in human and mouse genomes. Two homologous cDNA sequences in mouse and one in human have been cloned and their specific expression (mRNA) in epididymis has been shown. To study the functions, mouse and human BSP homologous proteins have been expressed in bacteria and purified. It has been shown that these proteins have several binding characteristics in common with their homologs previously studied and may also promote sperm capacitation. We are now interested in whether the deletion of these proteins in mouse (knock-out mouse) may have an effect on fertility and whether this protein could be a cause of infertility or subfertility in men. These studies may lead to a better understanding of the mechanisms involved in fertilization and identification of an important factor for human fertility.Mechanism of sperm protection by egg yolk and milk:Over the past 70 years, egg-yolk and milk have been routinely used in both liquid semen extenders and those used to cryopreserve sperm. However, the mechanism by which egg yolk and milk protect sperm during liquid storage or from freezing damage is unknown. Thus, our laboratory is studying the mechanisms involved in sperm protection by milk and egg yolk. We discovered that a group of phospholipid-binding proteins (PL-BP), which are detrimental to sperm, are added to sperm at ejaculation. Interestingly, these detrimental factors bind caseins/whey proteins and low-density lipoproteins found in milk and egg yolk respectively. This interaction appears to reduce lipid loss from the sperm membrane, thereby preventing membrane damage during storage and preserving the sperm functions (motility, viability and acrosomal integrity). In future studies we would like to further characterize the interaction between PL-BPs and milk/egg yolk components and develop novel sperm storage medium for commercial application.Affiliations and responsabilities
Research affiliations
Research units
Membre
Affiliated institutions
- CIUSSS de l'Est-de-l'Île-de-Montréal – Hôpital Maisonneuve-Rosemont (HMR)
Teaching and supervision
Student supervision
Theses and dissertation supervision (Papyrus Institutional Repository)
2021
A novel purification method for binder of SPerm proteins and characterization of the protein interaction network of BSPH1
Graduate : Sabouhi Zarafshan, Samin
Cycle : Doctoral
Grade : Ph. D.
Cycle : Doctoral
Grade : Ph. D.
2019
Knockout mouse model generated by CRISPR technology to study the function of BSP proteins on male fertility in vivo
Graduate : Eskandari Shahraki, Marzieh
Cycle : Doctoral
Grade : Ph. D.
Cycle : Doctoral
Grade : Ph. D.
2018
Binder of SPerm protein interference in sperm-egg interaction
Graduate : Heidari Vala, Hamed
Cycle : Master's
Grade : M. Sc.
Cycle : Master's
Grade : M. Sc.
2015
Rôle de protéines épididymaires humaines et murines dans les fonctions spermatiques
Graduate : Plante, Geneviève
Cycle : Doctoral
Grade : Ph. D.
Cycle : Doctoral
Grade : Ph. D.
2015
Fonction de la protéine Ceroid lipofuscinosis neuronal 5 (CLN5) dans le tri et le recyclage à l’endosome
Graduate : Jules, Felix
Cycle : Doctoral
Grade : Ph. D.
Cycle : Doctoral
Grade : Ph. D.
2011
Étude du mécanisme de protection des spermatozoïdes de mammifères par le lait
Graduate : Lusignan, Marie-France
Cycle : Doctoral
Grade : Ph. D.
Cycle : Doctoral
Grade : Ph. D.
2011
Le cancer épithélial des ovaires à cellules claires et de type mucineux exprime un niveau élevé de HYAL-1 : corrélation inverse avec l’expression des récepteurs d’estrogène et de progestérone
Graduate : Yoffou, Paule-Helena
Cycle : Master's
Grade : M. Sc.
Cycle : Master's
Grade : M. Sc.
2009
Les gènes et protéines BSP chez la souris et l’humain : clonage, caractérisation, expression sous forme recombinante et étude des fonctions biologiques
Graduate : Lefebvre, Jasmine
Cycle : Doctoral
Grade : Ph. D.
Cycle : Doctoral
Grade : Ph. D.
2007
Association des protéines du liquide séminal bovin aux membranes lipidiques par titrage calorimétrique isotherme, microscopie de fluorescence et la technique des monocouches
Graduate : Courtemanche, Lesley
Cycle : Master's
Grade : M. Sc.
Cycle : Master's
Grade : M. Sc.
2006
Induction de la capacitation des spermatozoïdes épididymaires porcins par pB1 et BSP-A1/-A2, des protéines de la famille des protéines BSP
Graduate : Lusignan, Marie-France
Cycle : Master's
Grade : M. Sc.
Cycle : Master's
Grade : M. Sc.
2006
Protéines du liquide séminal bovin et membranes lipidiques modèles : étude des interactions par titrage calorimétrique isotherme et spectroscopie infrarouge
Graduate : Lassiseraye, Danny
Cycle : Master's
Grade : M. Sc.
Cycle : Master's
Grade : M. Sc.
2005
Étude du mécanisme impliqué dans la protection des spermatozoïdes de mammifères par le jaune d'oeuf
Graduate : Bergeron, Annick
Cycle : Doctoral
Grade : Ph. D.
Cycle : Doctoral
Grade : Ph. D.
2004
Isolement de protéines liant les phospholipides à partir de tissus et du sérum bovins : évidences pour l'implication dans le transport inverse du cholestérol
Graduate : Lefebvre, Jasmine
Cycle : Master's
Grade : M. Sc.
Cycle : Master's
Grade : M. Sc.
2004
Caractérisation des protéines AQN-1 ET pB1 du plasma séminal porcin et leur rôle dans la capacitation des spermatozoïdes
Graduate : Crête, Marie-Hélène
Cycle : Master's
Grade : M. Sc.
Cycle : Master's
Grade : M. Sc.
1999
Isolement de protéines apparentées aux protéines majeures du plasma séminal bovin chez divers mammifères
Graduate : Ménard, Martin
Cycle : Master's
Grade : M. Sc.
Cycle : Master's
Grade : M. Sc.
1999
Étude de la capacitation des spermatozoïdes épididymaires bovins en présence des protéines majeures du plasma séminal bovin et des facteurs capacitants
Graduate : Thérien, Isabelle
Cycle : Doctoral
Grade : Ph. D.
Cycle : Doctoral
Grade : Ph. D.
1999
Clonage et distribution tissulaire des protéines majeures du plasma séminal bovin
Graduate : Salois, Dominic
Cycle : Master's
Grade : M. Sc.
Cycle : Master's
Grade : M. Sc.
1999
Le rôle d'une famille de protéines du plasma séminal bovin dans le transport lipidique et la capacitation du spermatozoïde
Graduate : Moreau, Robert
Cycle : Doctoral
Grade : Ph. D.
Cycle : Doctoral
Grade : Ph. D.
1998
Isolation et caractérisation de l'acétylhydrolase du facteur activateur de plaquettes du plasma séminal bovin
Graduate : Soubeyrand, Sébastien
Cycle : Doctoral
Grade : Ph. D.
Cycle : Doctoral
Grade : Ph. D.
1998
L'héparine et les lipoprotéines de haute densité induisent la capacitation des spermatozoïdes par des mécanismes différents
Graduate : Lane, Marie-Eve
Cycle : Master's
Grade : M. Sc.
Cycle : Master's
Grade : M. Sc.
Projects
Research projects
2017
- 2025
Réseau Québecois en Reproduction (RQR)
Lead researcher :
Derek Boerboom
Co-researchers :
Lawrence C. Smith
,
Puttaswamy Manjunath
,
Bruce D. Murphy
,
Christopher A. Price
,
Jacques Drouin
,
Jean-Claude Labbé
,
Julie Lavoie
,
Vardit Ravitsky
,
Jocelyn Dubuc
,
Greg Fitzharris
,
Kalidou Ndiaye
,
Sylvie Girard
,
Serge McGraw
,
Gustavo Zamberlam
,
Sophie Petropoulos
,
Guillaume St-Jean
,
Nicolas Gévry
,
Daniel J. Bernard
,
Cathy Vaillancourt
,
Jacquetta M Trasler
,
Hugh Clarke
,
Bernard Robaire
,
Guylain Boissonneault
,
Éric Asselin
,
Barbara F Hales
,
Sarah Kimmins
,
Rajesha Duggavathi
,
Carlos Reyes-Moreno
,
Marc-André Sirard
,
Robert Viger
,
François Richard
,
Janice L. Bailey
,
Pierre Leclerc
,
Robert Sullivan
,
Jacques J. Tremblay
,
Vilceu Bordignon
,
François Castonguay
,
Claude Robert
,
Vassilios Papadopoulos
,
Martine Culty
,
Yojiro Yamanaka
,
Daniel Cyr
,
Isabelle Plante
,
Géraldine Delbès
,
Clémence Belleannée
,
Nicolas Pilon
,
Rima Slim
,
Maritza Jaramillo Patino
,
Sylvie Breton
,
William Pastor
,
Céline Van Themsche
,
Véronique Ouellet
,
Simon Wing
,
Teruko Taketo-Hosotani
,
Aimee Ryan
,
Makoto Nagano
,
Daniel Dufort
,
Loydie Majewska
,
Cristian Marcel O'Flaherty
Funding sources:
FRQNT/Fonds de recherche du Québec - Nature et technologies (FQRNT)
Grant programs:
PVXXXXXX-(RS) Programme de regroupements stratégiques
2017
- 2023
Mechanism of mammalian sperm protection by extender components and development of novel extenders free of products of animal origin
Lead researcher :
Puttaswamy Manjunath
Funding sources:
CRSNG/Conseil de recherches en sciences naturelles et génie du Canada (CRSNG)
Grant programs:
PVX20965-(RGP) Programme de subvention à la découverte individuelle ou de groupe
2013
- 2019
BINDER OF SPERM (BSP) GENES AND PROTEINS : ROLE OF HUMAN AND MOUSE BSP PROTEINS IN SPERM FUNCTION AND FERTILIZATION
Funding sources:
IRSC/Instituts de recherche en santé du Canada
Grant programs:
PVXX5647-(MOP) Subvention de fonctionnement incluant les subventions de fonctionnement programmatiques (général)
2016
- 2018
A microcalorimetry system to define the biochemical parameters of molecular interactions
Lead researcher :
El Bachir Affar
Co-researchers :
Janos G. Filep
,
Puttaswamy Manjunath
,
Dindial Ramotar
,
Éric Milot
,
Przemyslaw (Mike) Sapieha
,
Sylvie Lesage
,
Jean-Sébastien Delisle
,
Frédérick Antoine Mallette
,
Hugo Wurtele
Funding sources:
CRSNG/Conseil de recherches en sciences naturelles et génie du Canada (CRSNG)
Grant programs:
PVXXXXXX-(OIR) Outils et d'instruments de recherche (de 7 001 $ à 150 000 $)
2016
- 2018
Mechanism of mammalian sperm protection by extender components and development of novel extenders free of products of animal origin
Lead researcher :
Puttaswamy Manjunath
Funding sources:
CRSNG/Conseil de recherches en sciences naturelles et génie du Canada (CRSNG)
Grant programs:
PVX20965-(RGP) Programme de subvention à la découverte individuelle ou de groupe
2004
- 2015
MAMMALIAN SPERM PRESERVATION : MECHANISM SPERM PROTECTION BY MILK AND DEVELOPMENT OF NOVEL EXTENDERS
Lead researcher :
Puttaswamy Manjunath
Funding sources:
CRSNG/Conseil de recherches en sciences naturelles et génie du Canada (CRSNG)
Grant programs:
PVX20965-(RGP) Programme de subvention à la découverte individuelle ou de groupe
2009
- 2013
DILUTERS OR EXTENDERS FOR MAMMALIAN SPERM PROCESSING AND PRESERVATION
Lead researcher :
Puttaswamy Manjunath
2009
- 2013
MAMMALIAN SPERM PRESERVATION: MECHANISM SPERM PROTECTION BY MILK AND DEVELOPMENT OF NOVEL EXTENDERS
Lead researcher :
Puttaswamy Manjunath
2008
- 2012
INSIGHTS INTO THE MECHANISMS UNDERLYING FERTILITY: ROLE OF NOVEL PHOSPHOLIPID-BINDING PROTEINS IN SPERM FUNCTION
Lead researcher :
Puttaswamy Manjunath
Outreach
Publications and presentations
Disciplines
- Biochemistry
Areas of expertise
- Nucleic Acids
- Cell
- Chromosome (Living Organisms)
- Enzymes and Proteins
- Gene (Living Organisms)
- Biological and Biochemical Mechanisms
- Bioactive Molecules