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Medical Sciences

Marcio Costa

L'interaction entre les animaux domestiques et leur microbiome.

Professeur adjoint

Faculté de médecine vétérinaire - Département de biomédecine vétérinaire

Pavillon 3200, rue Sicotte - Aile A, room 3980

514 343-6111 #37397

Secondary number: 450 773-8521 #37397 (Travail 1)


Awards and recognitions

XXIX World Buiatrics Congress Scholarship (2016)

Best research abstract ACVIM Forum (2013)

ESCMID Travel Grant (2010)

OVC PhD Fellowship (2010)

Best research abstract ACVIM Forum (2009)

XXV World Buiatrics Congress Scholarship (2008)

OVC'49 and Dr. Ray Cormack Graduate Entrance Scholarship (2008)

CNPq Master’s Fellowship (2006)


  • 2014 — PhD — — University of Guelph
  • 2010 — DVSc — — University of Guelph
  • 2003 — DVM — Médecine vétérinaireUniversity of Londrina

Affiliations and responsabilities

Professional titles and affiliations

Titles: corporations and professional organizations

Morris Animal Foundation (Large Animal Scientific Advisory Board)

Teaching and supervision


Research projects

2018 - 2024

Microbiota manipulation used to improve animal production

Lead researcher : Marcio Costa
Funding sources: CRSNG/Conseil de recherches en sciences naturelles et génie du Canada (CRSNG)
Grant programs: PVX20965-(RGP) Programme de subvention à la découverte individuelle ou de groupe
2020 - 2023

Utilisation de culturomics pour améliorer la santé intestinale chez les poulets

Lead researcher : Marcio Costa
2020 - 2023

Développement d'un écosystème bactérien pour améliorer les performances et la santé des poulets.

Lead researcher : Marcio Costa
Funding sources: FRQNT/Fonds de recherche du Québec - Nature et technologies (FQRNT)
Grant programs: PVXXXXXX-(NC) Établissement de la relève professorale
2020 - 2022

Detection of fecal microbiota imbalances to predict calf diarrhea

Lead researcher : Marcio Costa
Co-researchers : Sébastien Buczinski , Gilles Fecteau
Funding sources: Zoetis Canada Inc.
Grant programs:
2019 - 2022

Centre de Recherche en infectiologie porcine et avicole (CRIPA) / Regroupements stratégiques

Lead researcher : Carl A. Gagnon , Mariela Segura
Funding sources: FRQNT/Fonds de recherche du Québec - Nature et technologies (FQRNT)
Grant programs: PVXXXXXX-(RS) Programme de regroupements stratégiques
2019 - 2022

Improving Fecal Microbiota Transplantation in Horses.

Lead researcher : Marcio Costa , Luis Arroyo
Funding sources: Equine Guelph
Grant programs:
2019 - 2022

Improving Fecal Microbiota Transplantation in horses

Lead researcher : Luis Arroyo
Co-researchers : Marcio Costa
Funding sources: Equine Guelph
Grant programs:
2020 - 2021

Special Issue - Equine Microbiota

Lead researcher : Marcio Costa
2020 - 2021

Supplément COVID-19 CRSNG_Microbiota manipulation used to improve animal production

Lead researcher : Marcio Costa
Funding sources: CRSNG/Conseil de recherches en sciences naturelles et génie du Canada (CRSNG)
Grant programs: PVXXXXXX-Supplément à l’appui des étudiants, des stagiaires postdoctoraux et du personnel de soutien à la recherche COVID-19
2017 - 2020

Characterization of the anal sac microbiota in dog with sacculitis

Lead researcher : Marcio Costa
Funding sources: AKC Canine Health Foundation
Grant programs:


Publications and presentations


  1. LECLERE, M., COSTA MC, Fecal Microbiota in Equine Asthma, Journal of Veterinary Internal Medicine J Vet Intern Med. 2020 Mar;34(2):996-1006. doi: 10.1111/jvim.15748.
  2. The International Equine Colitis Research Group. Science-in-brief: Report on the Havemeyer Foundation workshop on acute colitis of the adult horse. Equine Veterinary Journal, 2020, 52: 163–164. DOI: 10.1111/evj.13223.
  3. BERGERON CC, COSTA MC, BERNARDI DE SOUZA L, SAUVÉ F, Characterization of bacterial microbiota of anal sacs in healthy dogs. Canadian Journal of Veterinary Research, 2020 (accepté).
  4. GOMEZ DE, GALVAO KN, RODRIGUEZ-LECOMPTE JC, COSTA MC. The cattle microbiota and the immune system: an evolving field. Veterinary Clinics of North America: Food Animal Practice, 2019, 35(3): 485-505. doi: 10.1016/j.cvfa.2019.08.002.
  5. COSTA MC, WESSE JS. Methods and Basic Concepts for Microbiota Assessment. The Veterinary Journal, 2019; 249: 10-15.
  6. ROMANA F, CLARK A, RUET A, COSTA MC, MACH N. Inter-breed diversity and temporal dynamics of the gut microbiota in healthy horses Journal of Animal Breeding and Genetics, 2019, doi: 10.1111/jbg.12441.
  7. KAMUS LJ, THEORET C, COSTA MC. Use of high throughput sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses. PlosOne. 2018 Nov 26;13(11):e0206989. doi: 10.1371/journal.pone.0206989
  8. COSTA MC, WESSE JS. Understanding the intestinal microbiome in health and disease. Veterinary Clinics of North America. Equine Practice. Advances in the Diagnosis and Management of Equine Gastrointestinal Disease, 2018, pii: S0749-0739(17)30918-5. doi: 10.1016/j.cveq.2017.11.005.
  9. PEREIRA, GQ, GOMES, LA, SANTOS, IF, ALFIERI, AF, WEESE, JS, COSTA, MC. Fecal Microbiota Transplantation in Puppies with Canine Parvovirus Infection. Journal of Veterinary Internal Medicine, 2018, published ahead. DOI: 10.1111/jvim.15072.
  10. ISHIKAWA AT, WEESE JS, BRACARENSE APFRL, ALFIERI AA, OLIVEIRA GG, KAWAMURA O, HIROOKA EY, ITANO EN, COSTA MC. Single aflatoxin B1 exposure induces changes in gut microbiota community in C57Bl/6 mice. World Mycotoxin Journal, 2017, v.10 (3) p.249-254. DOI:10.3920/WMJ2017.2190.
  11. ISHIKAWA AT, HIROOKA EY, ALVARES E SILVA PL, BRACARENSE APFRL, FLAIBAN KKMDC, AKAGI CY, KAWAMURA O, COSTA MC, ITANO EN. Impact of a Single Oral Acute Dose of Aflatoxin B₁ on Liver Function/Cytokines and the Lymphoproliferative Response in C57Bl/6 Mice. Toxins (Basel). 2017, v.9(11): E374. doi: 10.3390/toxins9110374.
  12. BESSEGATTO JA, PAULINO LR, LISBÔA JAN, ALFIERI AA, MONTEMOR CH, MEDEIROS LP, KOBAYASHI RKT, WEESE JS, COSTA MC Changes in the Fecal Microbiota of Beef Cattle Caused by Confinement and the Use of Virginiamycin as Growth Promoter. Research in Veterinary Science 2017, v.114, p.355-362.
  13. COSTA, MC; BESSEGATTO, JA; ALFIERI, AA; WEESE, JS; FILHO, JAB; OBA, A. Different antibiotic growth promoters induce specific changes in the cecal microbiota membership of broiler chicken. Plos One. 2017, v.12, e0171642
  14. ARROYO LG, COSTA MC, GUEST BB, PLATTNER BL, LILLIE BN, WEESE JS Experimental Model of Duodenitis-Proximal Jejunitis in Horses Inoculated with Clostridium difficile Toxins. Journal of Veterinary Internal Medicine, 2017; 31(1):158-163.
  15. GOMEZ, DE; ARROYO, LG; COSTA, MC; VIEL, L; WEESE, JS. Characterization of the Fecal Bacterial Microbiota of Healthy and Diarrheic Dairy Calves. Journal of Veterinary Internal Medicine, 2017 published ahead.
  16. ALMEIDA, MLM, FERINGER, WH, … COSTA, MC, FERRAZ, GC. Effects of Intense Exercise and Aerobic Conditioning Associated with Chromium or Carnitine Supplementation on the Fecal Microbiota of Fillies. Plos One 2016, v.11, e0167108.
  17. COSTA, MC, STÄMPFLI, HR, ALLEN-VERCOE, E, WEESE, JS. Development of the faecal microbiota in foals. Equine Veterinary Journal. 2015. doi: 10.1111/evj.12532.
  18. COSTA, MC, STÄMPFLI, HR, ARROYO, LG, ALLEN-VERCOE, E, GOMES, RG, WEESE, JS. Changes in the equine fecal microbiota associated with the use of systemic antimicrobial drugs. BMC Veterinary Research, 2015, v.11, e19.
  19. COSTA, MC, SILVA, G, RAMOS, RV, STÄMPFLI, HENRY R, ARROYO, LUIS G, WEESE, JS. Characterization and comparison of the bacterial microbiota in different gastrointestinal tract compartments in horses. The Veterinary Journal, 2015, v.205, 74-80.
  20. GORGIC, H., COSTA, MC, FRIENDSHIP R, CARMAN S, NAGY E. Genetic Characterization of H1N1 and H1N2 Influenza A Viruses Circulating in Ontario Pigs in 2012. Plos One, 2015, v.10, e0127840.
  21. GORGIC, H., COSTA, MC, FRIENDSHIP R, CARMAN S, NAGY E, WIDEMAN, G., WEESE, JS, POJAC, Z. Molecular characterization of H3N2 influenza A viruses isolated from Ontario swine in 2011 and 2012. Virology Journal, 2014, v.11, 194.
  22. STURGEON, A STULL, JW, COSTA, MC, WEESE, JS. Metagenomic analysis of the canine oral cavity as revealed by high-throughput pyrosequencing of the 16S rRNA gene In Veterinary Microbiology, 2013, v.162, 891-898.
  23. COSTA, MC, REID-SMITH, R, GOW, S., HANNON, S, BENEDICT, KM, MORLEY, PS, WEESE, JS. Prevalence and molecular characterization of Clostridium difficile isolated from feedlot beef cattle upon arrival and mid-feeding period. BMC Veterinary Research, 2012, v.8, e38.
  24. VETTORATO, ED, COSTA, MC, FLAIBAN, KKMC, VIDOTTO, O, BALARIN, MRS, LISBÔA, JAN. Serum proteins variation in Nelore and Holstein calves from birth to six months. Semina. Ciências Agrárias, 2012, v.33, 3181-3190.
  25. COSTA, MC, ARROYO, LG, ALLEN-VERCOE, E, STAEMPFLI, H, STURGEON, A., KIM, P, WEESE, JS. Comparison of the Fecal Microbiota of Healthy Horses and Horses with Colitis by High Throughput Sequencing of the V3-V5 Region of the 16S rRNA Gene. Plos One, 2012, v.7, e41484.
  26. COSTA, MC, WEESE, JS. The equine intestinal microbiome. Animal Health Research Reviews, 2012, v.1, 1-8.
  27. RUSH, S, PINDER, S, COSTA, MC, KIM, P. A Microbiology Primer for Pyrosequencing. Quantitative Bio-Science, 2012, v.31, 53-81.
  28. PARREIRA VR, COSTA, MC, EIKMEYER, F, BLOM, J, PRESCOTT, JF. Sequence of Two Plasmids from Clostridium perfringens Chicken Necrotic Enteritis Isolates and Comparison with C. perfringens Conjugative Plasmids. Plos One, 2012, v.7, e49753.
  29. STURGEON, A., PINDER SL, COSTA, MC, WEESE, JS. Characterization of the oral microbiota of healthy cats using next-generation sequencing. The Veterinary Journal, 2012, v.201, 223-229.
  30. ISERNHAGEN, AJ, CONSENZA, M, COSTA, MC, MÉDICI, KC, BALARIN, MRS, BRACARENSE, APFRL, ALFIERI, AA, LISBÔA, JAN. Asymptomatic encephalitis in calves experimentally infected with bovine herpesvirus-5. Canadian Veterinary Journal, 2011, v.52, 1312-1318.
  31. COSTA, MC, STAEMPFLI, H, ARROYO, LG, PEARL, DL, WEESE, JS. Epidemiology of Clostridium difficile on a veal farm: Prevalence, molecular characterization and tetracycline resistance. Veterinary Microbiology, 2011, v.152, 379-384.
  32. METCALF, D, COSTA, MC, DEW, WM, WEESE, JS. Clostridium difficile in vegetables, Canada. Letters in Applied Microbiology, 2010, v.51, 600-602.
  33. SANTOS, GMG, SILVA, KCF, CASIMIRO, TR, COSTA, MC, MORI, RM, MIZUBUTI, IY, MOREIRA, FB, SENEDA, MM. Reproductive performance of ewes mated in the spring when given nutritional supplements to enhance energy levels. Animal Reproduction, 2009, v.6, 422-427.
  34. COSTA, MC, FLAIBAN, KKMC, CONEGLIAN, MM, FEITOSA, FLF, BALARIN, MRS, LISBÔA, JAN. Passive transfer of immunity in Nelore and Limousin calves and serum proteinogram in the first four months of life. Brazilian Journal of Veterinary Research, 2008, v.28, 410-416.
  35. COSTA, MC, FLAIBAN, KKMC, CONEGLIAN, MM, DOGNANI, R, VETTORATO, ED, BALARIN, MRS, LISBÔA, JAN. Neutrophil oxidative burst in Nelore and Limousin calves in the first four months of life. Brazilian Journal of Veterinary Research, 2008, v.28, 431-436.
  36. COSTA, MC, ISERNHAGEN, AJ, MORAES, FLZ, DEARO, ACO, SAMPAIO, AJSA, REICHMANN, P, LISBÔA, JAN.. Absence of diarrhea in horses treated with oxytetracycline. Semina Ciência Agrárias, 2004, v.25, 245-248.


DIPIETRO R, COSTA MC. L’amélioration de la transplantation de microbiote fécal chez le cheval, La terre de chez nous (19/08/2019).

• COSTA MC, Current knowledge on Equine Microbiome, Proceedings of the XXVIII Plant and Animal Genomics, 2019, San Diego, USA.
• COSTA MC, ARROYO LG, SURETTE M. Microbiota manipulation in Horses: Current evidences and future directions. Havemeyer Equine Colic Workshop, août 2019, Gramby, Colorado, États Unis.
• COSTA MC The equine GI microbiome: what have we learned? Havemyer Workshop in Equine Colitis, July 2019, Niagara on the Lake, Canada.
• BERGERON CC, SAUVÉ F, BERNARDI DE SOUZA L, COSTA MC. Longitudinal characterization of anal sac microbiota in dogs with unilateral anal sacculitis treated with infusions of an antibiotic‐steroid‐antifungal suspension: a pilot study. Veterinary Dermatology, 2019; 30, 453–469, doi:10.1111/vde.12812.

• FRANCO LG, PARENT E, BOULIANNE M, BARJESTEH N, COSTA MC. Development of a protocol for colonization of the intestinal tract of chicks. 12th Annual Symposium du CRIPA, juin 2019, Montréal, Canada.

• GIROUX A, ZAMBERLAM G, ZANGITOLAMO A, SENEDA M, DROHER R, COSTA MC. Impact of estrous cycle on the vaginal microbiota and it’s association to pregnancy in dairy cows. 2019, Journée de la recherche (FMV), mars 2020, Saint-Hyacinthe, Canada (présentation orale).
• FRANCO L, BOULIANNE M, PARENT E, BARJESTEH N, COSTA MC Colonization of the Chickens’ Intestinal Tract Evaluated by Next Generation Sequencing , Journée de la recherche (FMV), mars 2020, Saint-Hyacinthe, Canada (présentation orale).
• DI PIETRO R, ARROYO L, LECLERE M, COSTA MC Evaluation of fecal microbiota transplantation in horses. Journée de la recherche (FMV), mars 2020, Saint-Hyacinthe, Canada (poster).
• FREZIER M, LABELLE E, OTIS C, LUSSIER B, BEAUDRY F, GAUDREAU P, TRONCY E, COSTA MC Comparison of intestinal microbiota of LOU/c/jall and Sprague-Dawley rats facing experimental osteoarthritis pain. Journée de la recherche (FMV), mars 2020, Saint-Hyacinthe, Canada (poster).


  • Veterinary Sciences
  • Biomedical Sciences

Areas of expertise

  • Microbiology
  • Bactériologie
  • Médecine interne des grands animaux
  • Animaux de la ferme
  • Animaux de compagnie