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Health Sciences; Medical Sciences; Natural Sciences and Engineering

Christine Vande Velde

Mécanismes cellulaires à l’origine de la sclérose latérale amyotrophique (SLA)

Professeure titulaire

Faculté de médecine - Département de neurosciences

c.vande.velde@umontreal.ca

Professeure accréditée

Faculté de médecine - Département de biochimie et médecine moléculaire

c.vande.velde@umontreal.ca

Secondary numbers: 514 890-8000 #28832 (Travail 1) 514 890-8000 #30229 (Travail 2)

Media

Travaillons ensemble à faire de la SLA une maladie traitable et non terminale

Portrait of Dr. Christine Vande Velde

Profile

Research expertise

Amyotrophic lateral sclerosis (ALS) is characterized by the selective loss of motor neurons. Motor neurons are unique in both their metabolic demand and architecture, and both are intricately linked. Efficient transport of cellular cargoes along the axonal process (which can be as much as one meter in length) depends on energy supplied by mitochondria. Furthermore, mitochondria are themselves cargos of axonal transport that must be delivered to the synapse so as to provide the necessary fuel for neurotransmission. Disease pathology includes disturbances in both of these elements, namely disrupted mitochondrial ultrastructure and axonal aggregates in both familial and sporadic ALS cases.

Mouse models confirm a defect in the axonal transport of at least one defined cargo, although it remains unknown if broader errors in intracellular trafficking exist. In addition, while various defects in mitochondrial metabolism have been reported, it remains unclear if these defects are unique to motor neurons and how they might participate in disease initiation. To examine how mitochondrial trafficking and axonal transport contribute to disease in vivo, we have generated a new transgenic mouse model in which mitochondria of motor neurons are fluorescently-labeled. These mice, in combination with rodents which develop an ALS-like phenotype, will be examined. Specifically, mitochondrial trafficking, dynamics, and axonal transport will be followed in relation to disease stage using confocal imaging of live motor axons. In addition, since mitochondria from motor neurons are labeled in these animals, motor neuron mitochondria will be isolated and assessed for multiple metabolic parameters. Complementary experiments in motor neuron cultures are also used.

Awards and recognitions

  • FRQS Junior 1
  • CIHR New Investigator
  • FRQS Senior

education

  • 1997 — BSc — GénétiqueUniversity of Manitoba
  • 2001 — PhD — BiochimieUniversity of Manitoba

Affiliations and responsabilities

Research affiliations

Teaching and supervision

Teaching

Student supervision

Theses and dissertation supervision (Papyrus Institutional Repository)

Projects

Research projects

2020 - 2026

UNDERSTANDING G3BP1 AND STRESS GRANULE DYNAMICS IN ALS PATHOGENESIS

Lead researcher : Christine Vande Velde
Funding sources: IRSC/Instituts de recherche en santé du Canada
Grant programs: PVXXXXXX-(PJT) Subvention Projet
2019 - 2023

Appréhender la colère sociale : une enquête comparative auprès des jeunes NEET au Québec et en Ontario

Lead researcher : Christine Vande Velde
Funding sources: CRSH/Conseil de recherches en sciences humaines du Canada
Grant programs: PVXXXXXX-Subvention Savoir
2015 - 2022

CROSS REGULATION OF RNA BINDING PROTEINS AND ORDERED PROTEIN AGGREGATION

Lead researcher : Christine Vande Velde
Funding sources: CRSNG/Conseil de recherches en sciences naturelles et génie du Canada (CRSNG)
Grant programs: PVX20965-(RGP) Programme de subvention à la découverte individuelle ou de groupe
2018 - 2021

DÉCRYPTER LES MÉCANISMES PATHOLOGIQUES DE LA NEURODÉGÉNÉRESCENCE DANS LA SCLÉROSE LATÉRALE AMYOTROPHIQUE

Lead researcher : Christine Vande Velde
Funding sources: FRQS/Fonds de recherche du Québec - Santé (FRSQ)
Grant programs: PVXXXXXX-Bourse de chercheur-boursier : Senior
2018 - 2020

Determining the conséquences of TDP-43 mediated splice event ind hnRNP a1

Lead researcher : Christine Vande Velde
Funding sources: ALS/Amyotrophic Lateral Sclerosis Association
Grant programs:
2018 - 2020

Disease relevance of hnRNP A1B, a TDP-43 dependent splice variant of HNRNPA1

Lead researcher : Christine Vande Velde
Funding sources: SLA Canada/Société canadienne de la sclérose latérale amyotrophique
Grant programs:
2015 - 2020

Regulation of the stress granule proteome and transcriptome by TDP-43 in ALS: biomarkers and therapeutic targets

Lead researcher : Christine Vande Velde
Funding sources: SLA Canada/Société canadienne de la sclérose latérale amyotrophique
Grant programs:
2002 - 2020

SUBVENTION D'INFRASTRUCTURE DU FRSQ POUR LE GRSNC(GROUPE DE RECHERCHE SUR LE SYSTÈME NERVEUX CENTRAL)

Funding sources: FRQS/Fonds de recherche du Québec - Santé (FRSQ)
Grant programs: PVXXXXXX-Subvention de groupe de recherche
2017 - 2019

High performance nucleofection unit for difficult to manipulate cell types

Lead researcher : Christine Vande Velde
Funding sources: CRSNG/Conseil de recherches en sciences naturelles et génie du Canada (CRSNG)
Grant programs: PVXXXXXX-(OIR) Outils et d'instruments de recherche (OIR) -1 -(de 7 001 $ à 150 000 $)
2016 - 2019

Misfolded SOD1 species and mitochondrial quality control in ALS

Lead researcher : Christine Vande Velde
Funding sources: Muscular Dystrophy Association
Grant programs:
2013 - 2018

EMERGING TEAM TO IDENTIFY AND CHARACTERIZE NOVEL AND EXISTING HEREDITARY SPASTIC PARAPELGIA (HSP) DISEASE GENES.

Lead researcher : Guy Rouleau
Co-researchers : Christine Vande Velde , Pierre Drapeau , Alex Parker
Funding sources: IRSC/Instituts de recherche en santé du Canada
Grant programs:
2013 - 2018

CELL BIOLOGICA MECHANISMS OF TDP-43 IN AMYOTROPHIC LATERAL SCLEROSIS

Lead researcher : Christine Vande Velde
Funding sources: IRSC/Instituts de recherche en santé du Canada
Grant programs: PVXXXXXX-Subvention de fonctionnement: recherche neuromusculaire
2013 - 2018

IMPACT OF TDP-43 ON STRESS GRANULE SIGNALING IN ALS ATION AND PROMOTE ISLET REGENERATION

Lead researcher : Christine Vande Velde
Funding sources: IRSC/Instituts de recherche en santé du Canada
Grant programs: PVXX5664-Bourse salariale pour nouveau chercheur
2015 - 2017

Misfolded SOD1 species in ALS pathogenesis

Lead researcher : Christine Vande Velde
Funding sources: SLA Canada/Société canadienne de la sclérose latérale amyotrophique
Grant programs:
2012 - 2017

CELL BIOLOGICAL MECHANISMS OF TDP-43 IN AMYOTROPIC LATERAL SCLEROSIS

Lead researcher : Christine Vande Velde
Funding sources: IRSC/Instituts de recherche en santé du Canada , SLA Canada/Société canadienne de la sclérose latérale amyotrophique
Grant programs: ,
2012 - 2017

EMERGING TEAM TO IDENTIFY AND CHARACTERIZE NOVEL AND EXISTING HEREDITARY SPASTIC PARAPLEGIA (HSP) DISEASE GENES

Lead researcher : Guy Rouleau
Funding sources: IRSC/Instituts de recherche en santé du Canada
Grant programs: PVXXXXXX-Subvention d'équipe émergente: maladies rares
2010 - 2016

ROLE OF TDP-43 IN CELLULAR STRESS RESPONSE

Lead researcher : Christine Vande Velde
Funding sources: CRSNG/Conseil de recherches en sciences naturelles et génie du Canada (CRSNG)
Grant programs: PVX20965-(RGP) Programme de subvention à la découverte individuelle ou de groupe
2012 - 2015

IMPACT OF TDP-43 ON STRESS GRANULE SIGNALING IN ALS

Lead researcher : Christine Vande Velde
Funding sources: Muscular Dystrophy Association
Grant programs:
2009 - 2014

ROLE OF TDP-43 IN CELLULAR STRESS RESPONSE

Lead researcher : Christine Vande Velde
2012 - 2013

FUNCTIONAL IMPLICATIONS OF MISFOLDED SOD1 ON MITOCHONDRIA IN ALS

Lead researcher : Christine Vande Velde
Funding sources: Bruno and Ilse Frick Foundation for Research on ALS
Grant programs:
2008 - 2013

CELLULAR AND BIOCHEMICAL IMPACT OF TDP-43 MUTANTS IN ALS

Lead researcher : Guy Rouleau
Co-researchers : Christine Vande Velde , Alex Parker , Jean-Pierre Julien
Funding sources: IRSC/Instituts de recherche en santé du Canada
Grant programs: PVXX5647-Subvention de fonctionnement incluant les subventions de fonctionnement programmatiques
2008 - 2011

IDENTIFICATION DES MECANISMES DE DEGENERESCENCE DU MOTONEURONE DANS LA SCLEROSE LATERALE AMYOTROPHIQUE (SLA)

Lead researcher : Christine Vande Velde

Outreach

Publications and presentations

Publications

Publications choisies

  • K.K. McDonald, A. Aulas, L. Destroismaisons, S. Pickles, E. Beleac, W. Camu, G.A. Rouleau, and C. Vande Velde. (2011). TAR DNA-Binding Protein 43 (TDP-43) regulates stress granule dynamics via differential regulation of G3BP and TIA-1. Human Molecular Genetics 20:1400-10;
  • C. Vande Velde#, K.K. McDonald, Y. Boukhedimi, M. McAlonis-Downes, C.S. Lobsiger, S. Bel Hadj, A.N. Zandona, J.P. Julien, S.B. Shah and D.W. Cleveland#. (2011). Misfolded SOD1 associated with motor neuron mitochondria alters mitochondrial shape and distribution prior to clinical onset. PLoS One 6:e22031;
  • S. Pickles and C. Vande Velde. (2012) Misfolded SOD1 and ALS: Zeroing in on mitochondria. Amyotrophic Lateral Sclerosis 13:333-40;
  • A. Aulas, S. Stabile and C. Vande Velde. (2012). Endogenous TDP-43, but not FUS, contributes to stress granule assembly via G3BP. Molecular Neurodegeneration 7:54;
  • S Pickles, L. Destroismaisons, S.L. Peyrard, S. Cadot, R.H. Brown Jr, G.A. Rouleau, J.P. Julien, N. Arbour and C. Vande Velde. (2013). Mitochondrial damage revealed by immunoselection for ALS-linked misfolded SOD1. Human Molecular Genetics 22:3947-39;
  • S. Pickles, M. Cadieux-Dion, J.I. Alvarez, M.A. Lécuyer, S. Peyrard, L. Destroismaisons, L. St.-Onge, S. Terouz, P. Cossette, A. Prat and C. Vande Velde. (2013). Endo-MitoEGFP mice: A novel transgenic mouse with fluorescently marked mitochondria in microvascular endothelial cells. PLOS One 8:e74603.

Disciplines

  • Neurosciences
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Areas of expertise

  • Multiple Sclerosis
  • Neurodegenerative Diseases (Aging)
  • Proteomics
  • Nucleic Acids