Philippe Roux et son équipe visent à caractériser les mécanismes de signalisation de plusieurs oncogènes et suppresseurs de tumeurs dans la régulation de la croissance et la prolifération cellulaire, en utilisant des techniques biochimiques et en biologie cellulaire, en génétique de la souris, en plus de méthodologies de pointes en protéomique, ils envisagent de découvrir de  meilleures cibles thérapeutiques contre le cancer.

Thèmes 

• Signalisation cellulaire
• Supresseur de tumeur
• Cancer
• Ras/MAPK
• mTOR
• Protéine kinase
• Oncogène
• Phosphorylation
• Protéomique

2020

NF45 and NF90 regulate mitotic gene expression by competing with Staufen-mediated mRNA decay

Nourreddine S, Lavoie G, Paradis J, Ben El Kadhi K, Meant A, Aubert L, Grondin B, Gendron P, Chabot B, Bouvier M, Carreno S, Roux PP

Cell Reports 31,107660 (2020)

Controversies around the function of LARP1

Berman AJ*, Thoreen CC*, Dedeic Z, Chettle J, Roux PP*, Blagden SP*

RNA Biol. 1, 1-11 (2020)

F-Actin interactome reveals Vimentin as a key regulator of Actin organization and cell mechanics in mitosis

Serres MP, Samwer M, Truong Quang BA, Petronczki M, Roux PP*, Paluch EK*

Dev. Cell 52, 210-222 (2020)

Loss of DP1 aggravates vascular remodeling in pulmonary arterial hypertension via mTORC1 signaling

He Y, Zuo C, Jia D, Bai P, Kong D, Chen D, Liu G, Li J, Wang Y, Chen G, Yan S, Xiao B, Zhang J, Piao L, Li Y, Deng Y, Li B, Roux PP, Andreasson KI, Breyer RM, Su Y, Wang J, Lyu A, Shen Y, Yu Y

Am. J. Respir. Crit. Care Med. 1, 1-15 (2020)

Proteomic analysis reveals a role for RSK in p120-catenin phosphorylation and melanoma cell-cell adhesion

Meant A, Gao B, Lavoie G, Nourreddine S, Jung F, Aubert L, Tcherkezian J, Gingras AC, Roux PP

Mol. Cell. Proteomics 19, 50-64 (2020)

2019

Regulation of protein kinase C delta Nuclear Import and Apoptosis by mTORC1

Layoun A, Goldberg AA, Baig A, Eng M, Attias O, Nelson K, Carella A, Amberber N, Fielhaber JA, Joung KB, Martin Schmeing T, Han Y, Downey J, Divangahi M, Roux PP, Kristof AS

Sci. Reports 9, 17620-33 (2019)

Human models of NUP98-KDM5A megakaryocytic leukemia in mice contribute to uncovering new biomarkers and therapeutic vulnerabilities

Cardin S, Bilodeau M, Roussy M, Aubert L, Milan T, Jouan L, Rouette A, Laramee L, Gendron P, Duchaine J, Decaluwe H, Spinella JF, Mourad S, Couture F, Sinnett D, Haddad E, Landry JR, Ma J, Humphries RK, Roux PP, Hebert J, Gruber TA, Wilhelm BT, Cellot S

Blood Advances 3, 3307-3321 (2019)

Misshapen coordinates protrusion restriction and actomyosin contractility during collective cell migration

Plutoni C, Keil S, Zeledon C, Delsin LEA, Decelle B, Roux PP, Carreno S, Emery G

Nature Comm. 10, 3940 (2019)

Mubritinib targets the electron transport chain complex I and reveals the landscape of OXPHOS dependency in acute myeloid leukemia

Baccelli I, Gareau Y, Lehnertz B, Gingras S, Spinella JF, Corneau S, Mayotte N, Girard S, Frechette M, Blouin-Chagnon V, Leveille K, Boivin I, MacRae T, Krosl J, Thiollier C, Lavallee VP, Kanshin E, Bertomeu T, Coulombe-Huntington J, St-Denis C, Bordeleau ME, Boucher G, Roux PP, Lemieux S, Tyers M, Thibault P, Hebert J, Marinier A, Sauvageau G

Cancer Cell 36, 84-99 (2019)

Predisposing germline mutations in high hyperdiploid acute lymphoblastic leukemia in children

de Smith AJ, Lavoie G, Walsh KM, Aujla S, Evans E, Hansen HM, Smirnov I, Kang AY, Zenker M, Ceremsak JJ, Stieglitz E, Muskens IS, Roberts W, McKean-Cowdin R, Metayer C, Roux PP, Wiemels JL

Genes Chromosomes Cancer 58, 723-730 (2019)

ERK2 regulates epithelial-to-mesenchymal plasticity through DOCK10-dependent Rac1/FoxO1 activation

Shin S, Buel GR, Nagiec MJ, Han MJ, Roux PP, Blenis J, Yoon SO

Proc. Natl Acad. Sci. USA. 116, 2967-2976 (2019)

2018

The Receptor Tyrosine Kinase AXL Is Required at Multiple Steps of the Metastatic Cascade during HER2-Positive Breast Cancer Progression

Goyette MA, Duhamel S, Aubert L, Pelletier A, Savage P, Thibault MP, Johnson RM, Carmeliet P, Basik M, Gaboury L, Muller WJ, Park M, Roux PP, Gratton JP, Côté JF

Cell Reports 23, 1476-1490 (2018)

Signaling pathways involved in the regulation of mRNA translation

Roux PP, Topisirovic I

Mol. Cell. Biol. 38, e00070-18 (2018)

RSK Regulates PFK-2 Activity to Promote Metabolic Rewiring in Melanoma

Houles T, Gravel SP, Lavoie G, Shin S, Savall M, Meant A, Grondin B, Gaboury L, Yoon SO, St-Pierre J, Roux PP

Cancer Res. 78, 2191-2204 (2018)

CdGAP/ARHGAP31 is regulated by RSK phosphorylation and binding to 14-3-3 adaptor protein

Ben Djoudi Ouadda A, He Y, Calabrese V, Ishii H, Chidiac R, Gratton JP, Roux PP, Lamarche-Vane N

Oncotarget 9, 11646-11664 (2018)

Defining the role of the RSK isoforms in cancer

Houles T, Roux PP

Semin. Cancer Biol. 48, 53-61 (2018)

2017

Focal Adhesion- and IGF1R-Dependent Survival and Migratory Pathways Mediate Tumor Resistance to mTORC1/2 Inhibition

Yoon SO, Shin S, Karreth FA, Buel GR, Jedrychowski MP, Plas DR, Dedhar S, Gygi SP, Roux PP, Dephoure N, Blenis J

Mol. Cell 67, 1-16 (2017)

Actin cortex architecture regulates cell surface tension

Chugh P, Clark A, Smith M, Cassani D, Dierkes K, Ragab A, Roux PP, Charras G, Salbreux G, Paluch E

Nature Cell. Biol. 19, 689-697 (2017)

EPCR expression marks UM171-expanded CD34+ cord blood stem cells

Fares I, Chagraoui J, Lehnertz B, MacRae T, Mayotte N, Tomellini E, Aubert L, Roux PP, Sauvageau G

Blood. 129, 3344-3351 (2017)

A new inhibitor of the B-arrestin/AP-2 endocytic complex reveals interplay between GPCR internalization and signalling

Beautrait A, Paradis JS, Zimmerman B, Armando S, Khoury E, Audet M, Roux PP, Laporte SA, Bouvier M

Nature Commun. 8, 15054 (2017)

ERK1/2 phosphorylate Gab2 to promote a negative feedback loop that attenuates PI3K/Akt signaling

Zhang X, Lavoie G, Meant A, Aubert L, Cargnello M, Haman A, Hoang T, Roux PP

Mol. Cell. Biol. 37, e00357-16 (2017)

Proteomics screen identifies class I Rab11 family interacting proteins as key regulators of cytokinesis

Laflamme C, Galan JA, Ben El Kadhi K, Meant A, Zeledon C, Carreno S, Roux PP*, Emery*

Mol. Cell. Biol. 37, e00278-16 (2017)

2016

High-throughput screening in niche-based assay identifies compounds to target preleukemic stem cells

Gerby B, Veiga DF, Krosl J, Nourreddine S, Ouellette J, Haman A, Lavoie G, Fares I, Tremblay M, Litalien V, Ottoni E, Kosic M, Geoffrion D, Ryan J, Maddox PS, Chagraoui J, Marinier A, Hebert J, Sauvageau G, Kwok BH, Roux PP, Hoang T.

J. Clin. Invest. 126, 4569-4584 (2016)

2015

Receptor sequestration in response to Beta-arrestin-2 phosphorylation by ERK1/2 governs steady-state levels of GPCR cell-surface expression

Paradis JS, Ly S, Blondel-Tepaza E, Galan JA, Beautrait A, Scott MGH, Enslen H, Marullo S, Roux PP*, Bouvier M*

Proc. Natl Acad. Sci. USA. 112, E5160-8 (2015)

Regulation of global and specific mRNA translation by the mTOR signaling pathway

Nandagopal N, Roux PP

Translation 3, e983402 (2015)

The expanding role of mTOR in cancer cell growth and proliferation

Cargnello M, Tcherkezian J, Roux PP

Mutagenesis 30, 169-176 (2015)

Effect of the transient pharmacological inhibition of mapk3/1 pathway on ovulation in mice

Siddappa D, Beaulieu E, Gevry N, Roux PP, Bordignon V, Duggavathi R

PLoS One 10, e0119387 (2015)

Translational control by oncogenic signaling pathways

Gao B, Roux PP

Biochim. Biophys. Acta. 1849, 753-765 (2015)

2014

Phosphoproteomic Analysis Identifies the Tumor Suppressor PDCD4 as a RSK Substrate Negatively Regulated by 14-3-3

Galan JA, Geraghty KM, Lavoie G, Kanshin E, Tcherkezian J, Calabrese V, Jeschkee GR, Turk BE, Ballif BA, Blenis J, Thibault P, Roux PP

Proc. Natl Acad. Sci. USA. 111, E2918-27 (2014)

A Biosensor to monitor Dynamic Regulation and Function of Tumour Suppressor PTEN in Living Cells

Lima-Fernandes E, Misticone S, Boularan C, Paradis JS, Enslen H, Roux PP, Bouvier M, Baillie GS, Marullo S, Scott MGH

Nat. Commun. 5, 4431 (2014)

Cellular control of cortical actin nucleation

Bovellan M, Romeo Y, Biro M, Boden A, Chugh P, Yonis A, Vaghela M, Fritzsche M, Moulding D, Thorogate R, Jegou A, Thrasher AJ,Romet-Lemonne G, Roux PP*, Paluch EK*, Charras G*

Curr. Biol. 24, 1628-1635 (2014)

Mechanistic target of rapamycin (mTOR) signaling during ovulation in mice

Siddappa D, Kalaiselvanraja A, Bordignon V, Dupuis L, Gasperin BG, Roux PP, Duggavathi R.

Mol Reprod Dev. 81, 655-665 (2014)

Mutations in DOCK7 in Individuals with Epileptic Encephalopathy and Cortical Blindness

Perrault I, Hamdan FF, Rio M, Capo-Chichi JM, Boddaert N, Decarie JC, Maranda B, Nabbout R, Sylvain M, Lortie A, Roux PP, Rossignol E, Gerard X, Barcia G, Berquin P, Munnich A, Rouleau GA, Kaplan J, Rozetemail JM, Michaud JL

Am J Hum Genet. 94, 1-7 (2014)

Proteomic analysis of cap-dependent translation identifies LARP1 as a key regulator of 5'TOP mRNA translation

Tcherkezian J, Cargnello M, Romeo Y, Huttlin EL, Lavoie G, Gygi SP, Roux PP

Genes & Dev. 28, 357-371 (2014)

Casein kinase 1e promotes cell proliferation by regulating mRNA translation

Shin S, Wolgamott L, Roux PP, Yoon SO

Cancer Res. 74, 201-211 (2014)

Glycogen synthase kinase-3β positively regulates protein synthesis and cell proliferation through the regulation of translation initiation factor 4E-binding protein 1

Shin S, Wolgamott L, Tcherkezian J, Vallabhapurapu S, Yu Y, Roux PP, Yoon SO.

Oncogene. 33, 1690-1699 (2014)

2013

The coming of age of phosphoproteomics; From large data sets to inference of protein functions

Roux PP, Thibault P

Mol. Cell. Proteomics. 12, 3453-3464 (2013)

Rapamycin resistance: mTORC1 substrates hold some of the answers

Yoon SO, Roux PP

Curr. Biol. 23, R880-R883 (2013)

Gab2 Phosphorylation by RSK Inhibits Shp2 Recruitment and Cell Motility

Zhang X, Lavoie G, Fort L, Huttlin EL, Tcherkezian J, Galan JA, Gu H, Gygi SP, Carreno S, Roux PP.

Mol. Cell. Biol. 33, 1657-1670 (2013)
 

Cell cortex composition and homeostasis resolved by integrating proteomics and quantitative imaging

Biro M, Romeo Y, Kroschwald S, Bovellan M, Boden A, Tcherkezian J, Roux PP*, Charras G*, Paluch E*

Cytoskeleton. 70, 741-754 (2013)

RSK promotes G2 DNA damage checkpoint silencing and participates in melanoma chemoresistance

Ray-David H, Romeo Y, Lavoie G, Deleris P, Tcherkezian J, Galan JA, Roux PP.

Oncogene. 32, 4480-4489 (2013)

Insulin activates RSK to trigger a new negative feedback loop that regulates insulin signaling for glucose metabolism

Smadja-Lamere N, Shum M, Deleris P, Roux PP, Abe JI, Marette A.

J. Biol. Chem. 288, 31165-31176 (2013)

Cell cycle regulation of Greatwall kinase localization facilitates mitotic progression

Wang P, Galan, JA, Bonneil E., Hickson GR, Roux PP, Thibault P, Archambault V.

J. Cell. Biol. 202, 277-293 (2013)

Role of TBC1D7, a subunit of the TSC1/TSC2 complex, in intellectual disability and megalencephaly

Capo-Chichi JM, Tcherkezian J, Decarie JC, Dobrzeniecka S, Patry L, Nadon MA, Mucha-Le Ny B, Shevell M, Cheikh BB, Joober R, Samuels ME, Rouleau GA, Hamdan FF, Roux PP, Michaud JL.

J. Med. Genet. 50, 740-744 (2013)

RSK regulates activated BRAF signalling to mTORC1 and promotes melanoma growth

Romeo Y, Moreau J, Zindy PJ, Saba-El-Leil M, Lavoie G, Dandachi F, Baptissart M, Borden KLB, Meloche S, Roux PP.

Oncogene. 32, 2917-26 (2013)