Pierre Thibault
Protéomique et spectrométrie bioanalytique
- Professeur titulaire
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Faculté des arts et des sciences - Département de chimie
Complexe des sciences
- Professeur accrédité
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Faculté de médecine - Département de biochimie et médecine moléculaire
Profile
Research expertise
Our research program focuses on the development and application of proteomics and bioanalytical mass spectrometry to the identification of trace-level proteins in complex cell extracts. Our research efforts bring a multidisciplinary team approach using methods and techniques from bioanalytical chemistry, protein chemistry, biochemistry and cellbiology.
More specifically, our research interests span from the development of cell fractionation methods and protein enrichment techniques for phospho- and glycoproteins to the coupling of multidimensional capillary liquid chromatography to mass spectrometry and its application to the identification of post-translational modifications (sites and stoechiometry). We are also developing chemometric and bioinformatics tools for data mining of peptides in complex protein digests. These different tools are used in proteomics research programs to gain further insights to complex cell biology paradigms such as organelle biogenesis (phagosome and endosome), cell signalling in cancer, and the identification of peptides presented by the major histocompatibility complexproteins.Biography
Pierre Thibault dirige l’unité de recherche en protéomique et spectrométrie de masse bioanalytique de l’IRIC et est professeur titulaire au Département de chimie de l’Université de Montréal.
Au cours de sa carrière, il a mis à profit son expertise en chimie bionalytique, en spectrométrie de masse et en protéomique pour nombre de projets de recherche multidisciplinaires, tout en repoussant les limites des technologies de ces domaines. Il collabore notamment avec plusieurs équipes de recherche de l’Institut, dont celles du Dr Claude Perreault et de Sébastien Lemieux, avec qui il a conçu une plateforme de découverte d’antigènes. Les travaux de son laboratoire ont également permis de développer des outils offrant une meilleure compréhension des mécanismes moléculaires impliqués dans l’immunité et la signalisation dans les cellules cancéreuses.
Il a débuté sa carrière au sein du Conseil national de recherches du Canada (CNRC) en tant que chercheur en chimie des protéines. Il y a entre autres mené des recherches sur la purification et la caractérisation des molécules provenant d’organismes marins.
En 2001, il est devenu directeur chez Caprion Pharmaceuticals, où il a travaillé à la conception d’une plateforme d’analyse des protéines et perfectionné les approches de protéomique quantitative pour l’identification et le profilage des protéines en faible concentration à la surface des cellules cancéreuses.
Il a ensuite joint l’Université de Montréal comme professeur titulaire et mis en place un programme de recherche sur le développement analytique en spectrométrie de masse et en protéomique.
Awards and recognitions
- 1988 : NRC President’s award for helping to solve a major national problem, following the seafood poisoning crisis of 1987.
- 1996 : NRC President’s outstanding award for analytical developments in biological mass spectrometry
- 2012 : Prix Maxxam en chimie analytique
- 2017 : Tony Pawson's award from the Canadian National Proteomic Network For his seminal contributions to mass spectrometry and proteomics
- 2018 - Prix Acfas Urgel-Archambault
Affiliations and responsabilities
Research affiliations
Teaching and supervision
Teaching
Courses taught (current session only)
- CHM-2102 – Chimie analytique 2
- CHM-2971 – Chimie bioanalytique
- CHM-3101 – Laboratoire de chimie analytique
- CHM-6140 – Spectrométrie de masse
Programs
- 106010 – Baccalauréat en chimie
- 146511 – Baccalauréat en biochimie et médecine moléculaire
- 148110 – Baccalauréat en santé publique environnementale et sécurité du travail
- 182810 – Baccalauréat en enseignement des sciences et des technologies au secondaire
- 206010 – Maîtrise en chimie
- 206013 – DESS en analyses environnementales et industrielles
Student supervision
Theses and dissertation supervision (Papyrus Institutional Repository)
Approche chimioprotéomique pour la déconvolution des cibles du MG624 dans les cellules AML
Cycle : Master's
Grade : M. Sc.
Proteogenomic analyses of colorectal cancer reveal tumor-specific antigens across microsatellite status
Cycle : Master's
Grade : M. Sc.
Optimization of differential ion mobility and segmented ion fractionation to improve proteome coverage
Cycle : Master's
Grade : M. Sc.
Synthèse de composés outils permettant l’approfondissement des connaissances biologiques dans le domaine oncologique
Cycle : Master's
Grade : M. Sc.
Quantitative proteomics identifies substrates of SUMO E3 ligase PIAS proteins involved in cell growth and motility
Cycle : Doctoral
Grade : Ph. D.
Régulation de la SUMOylation, de la phosphorylation et de l'ubiquitination en réponse au trioxyde d'arsenic
Cycle : Master's
Grade : M. Sc.
La maladie de Parkinson est-elle une maladie auto-immune ? À la recherche des acteurs moléculaires de la MitAP
Cycle : Master's
Grade : M. Sc.
Time-Resolved Phosphoproteomics Unravel the Dynamics of Intracellular Signaling
Cycle : Doctoral
Grade : Ph. D.
Study of a novel evolutionarily conserved pattern of histone acetylation
Cycle : Doctoral
Grade : Ph. D.
Dveloppement de nouvelles mthodes d'identification des sites de SUMOylation par protéomique
Cycle : Doctoral
Grade : Ph. D.
Quantitative proteomics methods for the analysis of histone post-translational modifications
Cycle : Doctoral
Grade : Ph. D.
Molecular characterization of the contribution of autophagy to antigen presentation using quantitative proteomics
Cycle : Doctoral
Grade : Ph. D.
Identification de nouveaux substrats des kinases Erk1/2 par une approche bio-informatique, pharmacologique et phosphoprotéomique
Cycle : Doctoral
Grade : Ph. D.
Étude de la voie de signalisation de l’insuline chez la drosophile par une approche phosphoprotéomique
Cycle : Doctoral
Grade : Ph. D.
Analytical strategies for the comprehensive profiling of histone post translational modifications by mass spectrometry and implications for functional analyses
Cycle : Doctoral
Grade : Ph. D.
Large scale identification of protein SUMOylation by mass spectrometry in HEK293 cells
Cycle : Master's
Grade : M. Sc.
Genèse de l’immunopeptidome du CMH de classe I
Cycle : Doctoral
Grade : Ph. D.
Mass spectrometry as a tool to dissect the role of chromatin assembly factors in regulating nucleosome assembly
Cycle : Doctoral
Grade : Ph. D.
Identification des peptides du complexe majeur d’histocompatibilité de classe I par spectrométrie de masse
Cycle : Master's
Grade : M. Sc.
Enrichissement de protéines ubiquitinées et une nouvelle approche protéomique pour l'identification des sites d'ubiquitination
Cycle : Master's
Grade : M. Sc.
Développement de méthodes analytiques pour la protéomique et l'identification de peptides MHC I issus de cellules leucémiques
Cycle : Doctoral
Grade : Ph. D.
Phosphoproteome profiling approaches for comprehensive monitoring of cell signaling events in interferon-[gamma] stimulated macrophages
Cycle : Master's
Grade : M. Sc.
Évaluation de différentes composantes chromatographiques d'un système nano-LC-MS pour des applications protéomiques
Cycle : Master's
Grade : M. Sc.
Module microfluidique intégrant des séparations multidimensionnelles : applications d'analyses protéomiques sur des extraits cellulaires
Cycle : Master's
Grade : M. Sc.
Mise au point d'un modèle d'étude des bases moléculaires de l'auto-renouvellement des cellules souches hématopoïétiques induit par HOXB4
Cycle : Master's
Grade : M. Sc.
Proteomic approaches for the detection of unusual post-translational modifications in simple and complex bacterial protein mixtures
Cycle : Doctoral
Grade : Ph. D.
Optimization of High Field Asymmetric Waveform Ion Mobility Spectrometry to enhance the comprehensiveness of mass spectrometry-based proteomic analyses
Cycle : Doctoral
Grade : Ph. D.
Other educational activities
- Professeur associé, Département d’anatomie et biologie cellulaire, Université McGill
Projects
Research projects
Uncovering tumor specific antigens and vulnerabilities in ETP-acute lymphoblastic leukemia
Characterization of endothelial cell death biomarkers for better prediction and prevention of renal fibrosis triggered by acute kidney injury
Next generation immunopeptidomics
Architecture and chemical modulation of genetic networks
PandemicStop-AI: for an accelerated response to pandemics
PandemicStop-AI: for an accelerated response to pandemics
Rôle des E3 SUMO ligases humaines de type PIAS dans le contrôle de l’expression des gènes
Neuroprotective peptides for treatment of neurological lysosomal diseases
RAS-MAPK signal transduction in normal and cancer cells
Time-of-Flight spectrometer for urgent resumption of accurate mass services.
Rational Design of Antigen-Targeted Breast Cancer Immunotherapy
Assessing the therapeutic potential of tumor-specific antigens for the treatment of lung and ovarian cancer / Évaluation du potentiel thérapeutique des antigènes spécifiques aux tumeurs pour le traitement des cancers pulmonaires et ovariens
The role of PD-related proteins as drivers of disease through modulation of innate and adaptive immunity
High resolution profiling of phosphoproteome dynamics in normal and mutant cells defective in mitotic kinase activity
Precision therapeutic vaccines against lung cancer
Characterization of endothelial cell death biomarkers for better prediction and prevention of renal fibrosis triggered by acute kidney injury
New proteomic methods to understand the regulation of ubiquitinlike modifiers in human diseases
High resolution profiling of phosphoproteome dynamics in normal and mutant cells defective in mitotic kinase activity
Uncovering Tumor Specific Antigens in T-cell Acute Lymphoblastic Leukemia
High sensitivity mass spectrometry platform for the discovery of tumor-specific antigens and the identification of prote
Neuroprotective peptides for treatment of neurological lysosomal diseases
Development of therapeutic vaccines against ovarian cancer - Développement de vaccins thérapeutiques contre le cancer ovarien
Development oftherapeutic vaccines against ovarian cancer - Développement de vaccins thérapeutiques contre le cancer ovarien
Centre for Advanced Proteomic and Chemogenomic Analyses (CAPCA)
The molecular heritage of vascular injury: Roles in rejection and renal failure
Discovery of actionable tumor-specific antigens in breast cancer Découverte d’antigènes spécifiques au cancer du sein/
Bioluminescence resonance energy transfer detection system for the monitoring of proteinprotein interactions, posttranslational modifications and cell signalling.
Développement de vaccins thérapeutiques contre les leucémies aigues myéloides et lymphoides / Oncopole
Systems biology of cell size homeostasis
Supplément COVID-19 CRSNG_New proteomic methods to understand the regulation of ubiquitinlike modifiers in human diseases
Uncovering Tumor Specific Antigens in ETP Acute Lymphoblastic Leukemia
Matching MHC 1-associated peptide spectra to sequencing reads using deep neural networks
The molecular heritage of vascular injury: Roles in rejection and renal failure
STRUCTURE DYNAMICS AND CONTINGENCY IN SIGNALING REGULATORY NETWORKS
MOLECULAR CHARACTERIZATION OF THE CONTRIBUTION OF AUTOPHAGY TO ANTIGEN PRESENTATION USING AN ITERATIVE PROTEOMICS APPROACH
MECHANISMS OF STABLE GENE REPRESSION BY THE PROMYELOCYTIC LEUKEMIA PROTEIN PML
Bridging the ProteoGenomics Gap for Personalized Medicine Using Transformative Mass Spectrometry Technologies
UNE APPROCHE CHIMIO- ET PROTEO-GENOMIQUE EN MEDECINE PERSONNALISEE POUR LA LEUCEMIE MYELOIDE AIGUE
NEW TECHNOLOGIES TO UNDERSTAND THE INTERPLAY OF PROTEIN MODIFICATIONS IN HUMAN DISEASES
ANTIGEN-SPECIFIC ADOPTIVE T CELL IMMUNOTHERAPY OF LEUKEMIA
Regulation of tumor supressor and E3 ligase FBW7 by the hormone-bound vitamin D receptor
The canadian National Transplant Research Program : Increasing donation and improving transplantation outcomes
PERSONALIZED CANCER IMMUNOTHERAPY PROGRAM
PERSONALIZED CANCER IMMUNOTHERAPY PROGRAM
THE CANADIAN NATIONAL TRANSPLANTATION RESEARCH PROGRAM (CNTRP) : INCREASING DONATION AND IMPROVING TRANSPLANTATION OUTCOMES - PROJECT 3
THE CANADIAN NATIONAL TRANSPLANTATION RESEARCH PROGRAM (CNTRP) ; INCREASING DONATION AND IMPROVING TRANSPLANTATION OUTCOMES - PROJECT 3
PROTEOMICS AND BIOANALYTICAL MASS SPECTROMETRY
Center for Advanced Proteomics Analyses (CAPA)
PERSONALIZED CANCER IMMUNOTHERAPY
DISCOVERY OF IMMUNODOMINANT MINOR HISTOCOMPATIBILI TY ANTIGENS (MIRA) IN HUMAN
PROHITS NEXT GENERATION : A FLEXIBLE SYSTEM FOR TRACKING ANALYZING AND REPORTING FUNCTIONNAL PROTEOMICS DATA
PROHITS NEXT GENERATION : A FLEXIBLE SYSTEM FOR TRACKING ANALYZING AND REPORTING
PROHITS NEXT GENERATION : A FLEXIBLE SYSTEM FOR TRACKING ANALYZING AND REPORTING
RESEAU POUR LES ETUDES THERAPEUTIQUES ET GENETIQUES DES CELLULES SOUCHES-STEMNET / NOVEL STRATEGIES TO EXPAND HUMAN HEMATOPOIETIC STEM CELL POPULATIONS FOR CLINICAL USE
LIPOLYSIS : BIOCHEMICAL GENETICS, PHYSIOLOGY AND MOLECULAR CIRCUITRY
STRUCTURE, DYNAMICS AND CONTINGENCY IN SIGNALING REGULATORY NETWORKS
THE PHAGOSOME PROTEOME AND SYSTEMS BIOLOGY : INSIGHTS INTO NOVEL PHAGOSOME FUNCTIONS
THOUSAND PROTEIN MULTIPLEXED MASS SPECTROMETRY ASSAY FOR BIOMARKER DISCOVERY (RENEWAL)
CREATION D'UN GROUPE D'ETUDE VISANT A MIEUX COMPRENDRE LA MALADIE DU GREFFON CONTRE L'HOTE CHRONIQUE (GVHC) ET DEVELOPPER DES BIOMARQUEURS POUR L'IDENTIFICATION PRECOCE DE LA MALADIE
ANTIGENES MINEURS D'HISTOCOMPATIBILITE IMMUNODOMINANTS - CIBLES DE L'IMMUNOTHERAPIE DU CANCER
CONTROL OF NEWLY SYNTHESIZED HISTONE DEACETYLATION AND ITS IMPLICATIONS FOR THE CLINICAL USE OF HDAC INHIBITORS
CHEMI-ENZYMATIC AND DATA MINING APPROACHES FOR THE IDENTIFICATION OF POST-TRANSLATIONAL MODIFICATIONS
PROTEOMICS; FROM PROTEIN STRUCTURES TO CLINICAL APPLICATIONS
Outreach
Publications and presentations
Publications
- J. Saba, E. Bonneil, C. Pomies, K. Eng, P.Thibault, “Enhanced Sensitivity in Proteomics Experiments Using FAIMS Coupled to a Hybrid Linear Ion Trap/Orbitrap Mass Spectrometer”, J. Proteome Res., 8, 3355–3366 (2009)
- P. Drogaris, Y. Le Blanc, J. Fitzgerald, N. Lowndes, A. Verreault, P. Thibault, “Enhanced protein detection using a novel trapping mode on a hybrid quadrupole-linear ion trap Q-Trap)”, Anal. Chem., 81, 6300-6309 (2009)
- M. Boutin, C. Berthelette, F. G. Gervais, M.-B. Scholand, J. Hoidal, M. F. Leppert, K. P. Bateman, P. Thibault, “High Sensitivity NanoLC-MS/MS Analysis of Urinary Desmosine and Isodesmosine”, Anal. Chem, 81, 1881-1887 (2009)
- M. Trost, L. English, M. Courcelles, M. Desjardins, P. Thibault, “Deciphering the Phagosomal Proteome in Interferon-g Activated Macrophages”, Immunity, 30, 143-154 (2009)
- Bendall S.C., Hughes C., Campbell J.L., Stewart MH, Pittock P., Liu S., Bonneil E., Thibault P., Bhatia M., Lajoie G.A., “An enhanced mass spectrometry approach reveals human embryonic stem cell growth factors in culture”, Mol. Cell. Proteomics, 8, 421-432 (2009)
- M. Gharib, M. Marcantonio, S.G. Lehmann, M. Courcelles, S. Meloche, A. Verreault, P. Thibault, “Artifactual O-sulfation of silver-stained proteins: Implications for the assignment of phosphorylation and sulfation sites”, Mol. Cell. Proteomics, 8, 506-518 (2009)
- P. Drogaris, H. Wurtele, H. Masumoto, A. Verreault, P. Thibault, “Comprehensive profiling of histone modifications using a label-free approach and its applications in determining structure-function relationships”, Anal. Chem, 80, 6698-6707 (2008)
- A. Carrière, M. Cargnello, L.-A. Julien, H. Gao, E. Bonneil, P. Thibault, P. P. Roux, “Oncogenic MAPK Signaling Regulates mTORC1 Assembly by Promoting Raptor Phosphorylation via ERK and RSK”, Current Biol, 18, 1269-1277 (2008)
- Y. Tang, M. A Holbert, H. Wurtele, K. Meeth, W. Rocha, M. Gharib, E. Jiang, P. Thibault, A.Verreault, P. A Cole, R. Marmorstein, “Fungal Rtt109 histone acetyltransferase is an unexpected structural homolog of metazoan p300/CBP”, Nature Struct. Mol. Biol., 15, 738-745 (2008)
- M. Marcantonio, M. Trost, M. Courcelles, M. Desjardins, P. Thibault, “Combined enzymatic and data mining approaches for comprehensive phosphoproteome analyses; application to cell signaling events of interferon-γ stimulated macrophages”, Mol Cell Proteomics, 7, 645-660 (2008)
- M.-H. Fortier, E. Caron, M.-P. Hardy, G. Voisin, S. Lemieux, C. Perreault, P. Thibault, “The MHC I immunopeptidome is moulded by the transcriptome”, J. Exp. Med., 205, 595-610 (2008)
- C.A. Smith, K.M. Lau, Z. Rahmani, S. E. Dho, G. Brothers, Y. Min She, D. M. Berry, E. Bonneil, P. Thibault, F. Schweisguth, R. Le Borgne, C.J. McGlade, “aPKC-mediated phosphorylation regulates asymmetric membrane localization of the cell fate determinant Numb”, EMBO J., 26, 468-480 (2007)
Disciplines
- Chemistry
- Biochemistry
Areas of expertise
- Bioanalytical chemistry
- Proteomics
- Spectroscopy
- Mass spectrometry
- Proteins
- Protein modifications
- Cell signalling
- Analytical chemistry